Identification of collagen genes related to immune infiltration and epithelial-mesenchymal transition in glioma

Abstract Background Gliomas account for the majority of fatal primary brain tumors, and there is much room research in underlying pathogenesis, multistep progression glioma, how to improve survival. In our study, we aimed identify potential biomarkers or therapeutic targets glioma study mechanism tumor progression. Methods We downloaded microarray datasets (GSE43378 GSE7696) from Gene Expression Omnibus (GEO) database. Then, used weighted gene co-expression network analysis (WGCNA) screen related ESTIMATE (Estimation STromal Immune cells MAlignant Tumors using data) algorithm TIMER (Tumor Estimation Resource) database were analyze correlation between selected genes microenvironment. Real-time reverse transcription polymerase chain reaction was measure gene. Transwell wound healing assays cell migration invasion capacity. Western blotting test expression epithelial-mesenchymal transition (EMT) markers. Results identified specific module that positively correlated with WHO grade but negatively OS glioma. Importantly, 6 collagen (COL1A1, COL1A2, COL3A1, COL4A1, COL4A2, COL5A2) could regulate immunosuppressive microenvironment Moreover, found these significantly involved EMT process Finally, taking COL3A1 as a further object, results showed knockdown inhibited migration, invasion, SHG44 A172 cells. Conclusions summary, demonstrated play an important role regulating serve management.